Hydrogen Peroxide (H2O2) Assay

This kit is designed to test for hydrogen peroxide content of aqueous biological samples.

Recommended for researchers wishing to assay H₂O₂ in tissue homogenates, cell lysates & other biological fluids or extracts where H₂O₂ is present.

Summary

Hydrogen Peroxide (H₂O₂) is a highly reactive species normally produced in cells by both enzymatic and non-enzymatic pathways. The reaction of superoxide disputase (SOD) with superoxide radicals within cells and in surrounding areas results in the uncontrolled formation of H₂ O₂. Granulocytes also produce H₂O₂ via metabolic processes as an immunological response to foreign invaders. While the metabolic production of H₂O₂ by granulocytes is controlled at the immunological level, the production of H₂O₂ as a by-product of oxidative stres is not wellregulated and thus cells must rely on the enzymes catalase and glutathione peroxidase to convert H₂O₂ into harmless water and oxygen. As a by-product of the SOD oxidative stress response and as an integral part of normal inflammatory response to infection, understanding the impact of H₂O₂ on various model systems hes become an important facet of studying many pathophysiological processes.

The NWLSS™ Hydrogen Peroxide assay is based on the oxidation of ferrous ions to ferric ions by peroxides wherein the ferric ions react with the indicator dye xylenol orange to produce a purple colored complex measureable at 560-590 nm. Sorbitol is included to amplify the color intensity. Reaction of samples with and without catalase enzyme provides the means to specifically quantify H₂O₂ by difference in sample unknowns.